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Sci.Rep:华中农业大学李大鹏研究发表黄鳝性逆转机制研究进展

摘要 : 近日,自然出版集团旗下综合类刊物《Scientific Reports》在线发表华中农业大学水产学院渔业资源与环境团队李大鹏研究员黄鳝性逆转机制的研究新进展

 

近日,自然出版集团旗下综合类刊物《Scientific Reports》在线发表华中农业大学水产学院渔业资源与环境团队李大鹏研究员黄鳝性逆转机制的研究新进展,论文题为“Molecular cloning and characterization of amhand dax1 genes and their expression during sex inversion in rice-field eel Monopterus albus”。胡庆为论文第一作者,李大鹏研究员为论文通讯作者。

黄鳝(Monopterus albus)为温热带淡水鱼类,具有由雌到雄转变的性逆转现象。对黄鳝性逆转机制的研究不仅对阐明黄鳝的发育机制具有重要理论意义,而且对黄鳝的科学养殖具有重要的应用价值。研究发现,amh基因在性腺中的表达量表现为雄性>间性>雌性,表明该基因的上调能够启动黄鳝雄性支持细胞的分化,并且对黄鳝雄性的发育和精巢的维持具有一定的作用。

dax1不管是在雌性、间性和雄性都有高水平的表达,表明它与黄鳝卵巢的发育以及精巢的发育和维持有关。另外,它们都可以通过直接或间接调控cyp19a1amRNA的表达来调控性腺的发育。此外,dax1基因广泛分布在包括性腺在内的多个组织中,但amh只局限在性腺中表达,表明该基因对性腺的发育和分化具有重要作用。该研究对amh和dax1表达模式以及与性逆转调控关系的解释,为阐释黄鳝的性逆转机制奠定了理论基础。

原文链接:

Molecular cloning and characterization of amhand dax1 genes and their expression during sex inversion in rice-field eel Monopterus albus

原文摘要:

The full-length cDNAs of amh and dax1 in the hermaphrodite, rice-field eel (Monopterus albus), were cloned and characterized in this study. Multiple sequence alignment revealed Dax1 was well conserved among vertebrates, wheras Amh had a low degree of similarity between different vertebrates. Their expression profiles in gonads during the course of sex inversion and tissues were investigated. The tissue distribution indicated amh was expressed mostly in gonads and was scarcely detectable in other tissues, wheras the expression of dax1 was widespread among the different tissues, especially liver and gonads. amh was scarcely detectable in ovaries wheras it was abundantly expressed in both ovotestis and testis. By contrast, dax1 was highly expressed in ovaries, especially in ♀IV (ovaries in IV stage), but it was decreased significantly in ♀/♂I (ovotestis in I stage). Its expression was increased again in ♀/♂III (ovotestis in III stage), and then decreased to a low level in testis. These significant different expression patterns of amh and dax1 suggest the increase of amh expression and the decline ofdax1 expression are important for the activation of testis development, and the high level of amh and a low level of dax1 expression are necessary for maintenance of testis function.

来源: Scientific Reports 浏览次数:0

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