nature

当前位置: Nature » 动物学 » 正文

Sci.Rep:农科院畜牧兽医所张宏福团队揭示肉鸡热应激调控机制

摘要 : 2015年10月13日,自然出版集团旗下期刊《Scientific Reports》上发表了家畜营养与调控创新团队揭示肉鸡热应激调控机制,家畜营养与调控创新团队利用蛋白质组定量技术解析热应激下肉鸡体内蛋白质变化趋势

 2015年10月13日,自然出版集团旗下期刊《Scientific Reports》上发表了家畜营养与调控创新团队揭示肉鸡热应激调控机制,家畜营养与调控创新团队利用蛋白质组定量技术解析热应激下肉鸡体内蛋白质变化趋势,并鉴定到多个参与肉鸡热应激调控的关键蛋白,从蛋白质水平上揭示了肉鸡热应激调控机制。

在集约化养殖的环境下,热应激已成为现代家禽生产中普遍关注的全球性问题。热应激可导致肉鸡的生长速度变慢、饲料转化率低,肉品质变差等不利影响,并且严重影响肉鸡免疫力甚至导致肉鸡死亡率,给肉鸡生产带了巨大经济损失。

家畜营养与调控创新团队致力于研究动物生长环境-营养-健康之间的协同调控关系,此次利用非数据依赖采集模式下(Data-independent acquisition, DIA)的蛋白质非标记蛋白质定量技术(SWATH)分析热应激下肉鸡肝脏内蛋白质的变化,共鉴定到257个显著差异蛋白,这些蛋白广泛参与氧化还原反应,氨基酸代谢,脂肪代谢等生物调控过程。通过生物信息学深度挖掘蛋白功能发现,热应激下肉鸡主要通过抑制细胞外信号调节激酶(Extracellular signal-regulated protein kinases,ERK)通路影响肝细胞凋亡;通过增强甲硫氨酸的体内合成增强机体抗氧化能力;通过增加儿茶酚胺类化合物加快呼吸频率以达到降温的目的。该研究初步揭示了高温下肉鸡机体的影响和肉鸡自我免疫调控机制,有助于今后通过遗传育种改良和改善营养配方提高肉鸡对热应激的适应能力。

原文链接:

Label-free Quantitative Analysis of Changes in Broiler Liver Proteins under Heat Stress using SWATH-MS Technology

原文摘要:

High temperature is one of the key environmental stressors affecting broiler production efficiency and meat yield. Knowledge of broiler self-regulation mechanisms under heat stress is important for the modern scale of poultry breeding. In the present study, the SWATH strategy was employed to investigate the temporal response of the broiler liver to heat stress. A total of 4,271 proteins were identified and used to generate a reference library for SWATH analysis. During this analysis, 2,377 proteins were quantified, with a coefficient of variation ≤25% among technical and biological replicates. A total of 257 proteins showed differential expression between the control and heat stressed groups. Consistent results for 26 and 5 differential proteins were validated respectively by MRM and western blotting quantitative analyses. Bioinformatics analysis suggests that the up- and down-regulation of these proteins appear involved in the following three categories of cellular pathways and metabolisms: 1) inhibit the ERK signaling pathway; 2) affect broiler liver lipid and amino acid metabolism; 3) induce liver cell immune responses to adapt to the high temperatures and reduce mortality. The study reported here provides an insight into broiler self-regulation mechanisms and shed light on the improved broiler adaptability to high-temperature environments.

来源: Scientific Reports 浏览次数:0

热门文章TOP

RSS订阅 - 填写您的邮件地址,订阅我们的精彩内容: - 网站地图
网站联系电话:020-87540820 备案号:粤ICP备11050685号-8 增值电信业务经营许可证:粤B2-20120479
©2011-2015 生物帮 All rights reserved.