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Sci Rep:南京师范大学尹绍武课题组发表瓦氏黄颡鱼低氧应激及种质改良研究进展

摘要 : 2016年3月10日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志上在线发表南京师范大学生命科学学院尹绍武教授课题组在瓦氏黄颡鱼低氧应激及遗传育种的研究中取得系列研究成果。

 2016年3月10日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志上在线发表南京师范大学生命科学学院尹绍武教授课题组在瓦氏黄颡鱼低氧应激及遗传育种的研究中取得系列研究成果。研究成果题为“Integrated analysis of mRNA-seq and miRNA-seq in the liver of Pelteobagrus vachelli in response to hypoxia”。,其他相关成果在《Chemosphere》(SCI IF=3.34)等SCI期刊发表,累积影响因子13.88(2015年),系列论文的第一作者均为生科院2014级博士生张国松。同时,课题组在瓦氏黄颡鱼种质改良方面,培育出比瓦氏黄颡鱼耐低氧的杂交黄颡鱼新品系。

与陆生环境相比,水环境呈现出更为宽广的溶氧含量的时空变化,因此,相比鸟类和哺乳类,终生生活于水体的鱼类能适应更为宽广的氧环境。由于在长期演化历程中受到不同浓度溶氧水体的自然选择,鱼类形成了适应不同溶氧水环境的物种。即使是在同一海拔高度水体分布的近缘物种间或同一物种的不同品系间,它们对水体溶氧的适应力也存在显著差异。此外,对于分布于不同海拔高度水体的近缘物种间或不同品系间,它们对水体溶氧适应能力的差异更大。对于鱼类低氧适应机制的研究,不仅在认识鱼类物种的分化以及低氧信号传导途径的系统演化和调控等方面具有十分重要的理论意义,而且对于培育耐低氧鱼类新品种具有十分重要的实践意义。针对这一现状,尹绍武教授课题组首次对鱼类低氧应激下差异转录组与差异miRNA组进行联合分析,结合检测抗氧化物酶系统、氧传感蛋白、呼吸代谢系统等指标变化,探究了鱼类低氧适应机制,研究结果为杂交黄颡鱼新品种的培育奠定了理论基础。

原文链接:

Integrated analysis of mRNA-seq and miRNA-seq in the liver of Pelteobagrus vachelli in response to hypoxia

原文摘要:

Pelteobagrus vachelli is a well-known commercial species in Asia. However, a sudden lack of oxygen will result in mortality and eventually to pond turnover. Studying the molecular mechanisms of hypoxia adaptation in fishes will not only help us to understand fish speciation and the evolution of the hypoxia-signaling pathway, but will also guide us in the breeding of hypoxia-tolerant fish strains. Despite this, the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in hypoxia responses in fish have remained unexamined. In the present study, we used next-generation sequencing technology to characterise mRNA-seq and miRNA-seq of control- and hypoxia-treated P. vachelli livers to elucidate the molecular mechanisms of hypoxia adaptation. We were able to find miRNA-mRNA pairs using bioinformatics analysis and miRNA prediction algorithms. Furthermore, we compared several key pathways which were identified as involved in the hypoxia response of P. vachelli. Our study is the first report on integrated analysis of mRNA-seq and miRNA-seq in fishes and offers a deeper insight into the molecular mechanisms of hypoxia adaptation. qRT-PCR analysis further confirmed the results of mRNA-Seq and miRNA-Seq analysis. We provide a good case study for analyzing mRNA/miRNA expression and profiling a non-model fish species using next-generation sequencing technology.

来源: Scientific Reports 浏览次数:0

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