nature

当前位置: Nature » 分子生物学 » 正文

Nat Protoc:南京大学刘震教授研究团发表分子印迹研究论文

摘要 : 2017年4月6日,国际学术权威刊物自然出版集团旗下子刊《Nature Protocols》在线发表了南京大学化学化工学院刘震教授研究团队关于分子印迹领域的重要论文

 2017年4月6日,国际学术权威刊物自然出版集团旗下子刊《Nature Protocols》在线发表了南京大学化学化工学院刘震教授研究团队关于分子印迹领域的重要论文,研究论文题为“Preparation of molecularly imprinted polymers specific to glycoproteins, glycans and monosaccharides via boronate affinity controllable-oriented surface imprinting”。论文的共同第一作者为博士生邢荣荣和博士后王双寿。

分子印迹是制备具有类似抗体或酶专一性仿生识别材料的重要技术,在生物传感、亲和分离和疾病诊断等领域具有广阔的应用前景。但是,蛋白质等生物分子的普适、高效印迹是分子印迹中的一个重要的挑战,传统方法难以同时满足不同大小的生物分子的印迹,更难以对印迹过程进行精确控制。经过多年在硼亲和材料领域中的深入研究,该研究团队发展出硼亲和可控定向表面印迹法。该方法能够便捷高效地制备出糖蛋白、聚糖和单糖的分子印迹聚合物。该方法的原理见图1,先将模板分子固定到硼酸功能化的基质上,然后利用生物相容性良好的功能单体在基质表面聚合,形成合适厚度的印迹层以及与模板分子空间匹配的印迹腔。该方法的最大优势在于印迹可控性:根据模板分子尺寸大小,调整印迹层厚度,制备出不同尺寸模板的分子印迹聚合物,而印迹层厚度只需要调节印迹时间来精确控制。这不仅简化了印迹步骤,而且适用范围广。所得分子印迹聚合物已经在疾病诊断、癌细胞靶向识别和活体单细胞分析等重要应用领域中展现出优越的分子识别性能。

图1. 硼亲和可控定向表面印迹原理

原文链接:

Preparation of molecularly imprinted polymers specific to glycoproteins, glycans and monosaccharides via boronate affinity controllable-oriented surface imprinting

原文摘要:

Molecularly imprinted polymers (MIPs) are materials that are designed to be receptors for a template molecule (e.g., a protein). They are made by polymerizing the polymerizable reagents in the presence of the template; when the template is removed, the material can be used for many applications that would traditionally use antibodies. Thus, MIPs are biomimetic of antibodies and in this capacity have found wide applications, such as sensing, separation and diagnosis. However, many imprinting approaches are uncontrollable, and facile imprinting approaches widely applicable to a large variety of templates remain limited. We developed an approach called boronate affinity controllable–oriented surface imprinting, which allows for easy and efficient preparation of MIPs specific to glycoproteins, glycans and monosaccharides. This approach relies on immobilization of a template (glycoprotein, glycan or monosaccharide) on a boronic-acid-functionalized substrate through boronate affinity interaction, followed by self-polymerization of biocompatible monomer(s) to form an imprinting layer on the substrate with appropriate thickness. Imprinting in this approach is performed in a controllable manner, permitting the thickness of the imprinting layer to be fine-tuned according to the molecular size of the template by adjusting the imprinting time. This not only simplifies the imprinting procedure but also makes the approach widely applicable to a large range of sugar-containing biomolecules. MIPs prepared by this approach exhibit excellent binding properties and can be applied to complex real samples. The MIPs prepared by this protocol have been used in affinity separation, disease diagnosis and bioimaging. The entire protocol, including preparation, property characterization and performance evaluation, takes ~3–8 d, depending on the type of substrate and template used.

来源: Nature Protocols 浏览次数:0

热门文章TOP

RSS订阅 - 填写您的邮件地址,订阅我们的精彩内容: - 网站地图
网站联系电话:020-87540820 备案号:粤ICP备11050685号-8 增值电信业务经营许可证:粤B2-20120479
©2011-2015 生物帮 All rights reserved.