nature

当前位置: Nature » 微生物学 » 正文

Sci Rep:兰州大学李红玉教授课题组发表微生物制药研究成果

摘要 : 2月4日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线刊登了兰州大学李红玉教授课题组在微生物制药方面的研究成果。

 2月4日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线刊登了兰州大学李红玉教授课题组在微生物制药方面的研究成果。论文题为“A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid”,文章第一作者为药学院陈朋副研究员,通讯作者为李红玉教授。

近年来,Vanillin已经成为合成许多重要药物的来源或中间体:如治疗帕金森氏病药物的中间体,治疗上呼吸道感染和防止性病菌传播药物的中间体,治疗心脏病药物的中间体,以及治疗肿瘤、神经性疼痛的药物等。但长期以来天然Vanillin原料匮缺,其安全有效的微生物合成方法成为国际医药界关注的热点。

研究组利用自然界普遍存在的Ferulic acid为底物来源,将微生物转化技术用于制备Vanillin。通过筛选、测定不同来源的Vanillin生产菌株的Ferulic acid转化能力,从中获得一株耐受高浓度Ferulic acid的、具有稳定转化能力的枯草芽孢杆菌,并在多批次发酵实验基础上获得了中试关键参数,通过生物转化成功将底物转化为Vanillin。论文还探讨了微生物在Ferulic acid转化Vanillin过程中的环境适应机制。

原文链接:

A microbial transformation using Bacillus subtilis B7-S to produce natural vanillin from ferulic acid

原文摘要:

Bacillus subtilis strain B7-S screened from18 strains is an aerobic, endospore-forming, model organism of Gram-positive bacteria which is capable to form vanillin during ferulic acid bioconversion. The bioconversion of ferulic acid to vanillin by Bacillus subtilis B7-S (B. subtilis B7-S) was investigated. based on our results, the optimum bioconversion conditions for the production of vanillin by B. subtilis B7-S can be summarized as follows: temperature 35 °C; initial pH 9.0; inoculum volume 5%; ferulic acid concentration 0.6 g/L; volume of culture medium 20%; and shaking speed 200 r/min. Under these conditions, several repeated small-scale batch experiments showed that the maximum conversion efficiency was 63.30% after 3 h of bioconversion. The vanillin products were confirmed by spectral data achieved from UV–vis, inductively coupled plasma atomic emission spectroscope (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) spectra. Scanning electron microscopy (SEM) and transmission electron spectroscopy (TEM) results confirmed that the cell surface ofB. subtilis plays a role in the induction of ferulic acid tolerance. These results demonstrate that B. subtilis B7-S has the potential for use in vanillin production through bioconversion of ferulic acid.

来源: Scientific Reports 浏览次数:0

热门文章TOP

RSS订阅 - 填写您的邮件地址,订阅我们的精彩内容: - 网站地图
网站联系电话:020-87540820 备案号:粤ICP备11050685号-8 增值电信业务经营许可证:粤B2-20120479
©2011-2015 生物帮 All rights reserved.