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Nature:南开联合研究团队在表界面修饰的研究领域获进展

标签:绿原酸 消毒
摘要 : 近日,我校生命科学学院、药物化学生物学国家重点实验室冯喜增教授的联合研究团队(南开生命科学院、南开大学计算机与控制工程学院、波兰科学院物理化学研究所等)在表/界面修饰的研究领域的一项研究成果发表于Nature出版集团《Scientific Reports》杂志上。

 近日,我校生命科学学院、药物化学生物学国家重点实验室冯喜增教授的联合研究团队(南开生命科学院、南开大学计算机与控制工程学院、波兰科学院物理化学研究所等)在表/界面修饰的研究领域的一项研究成果发表于Nature出版集团《Scientific Reports》杂志上。任崧博士、博士生吴明、硕士生郭伽瑜是本文的并列第一作者,南开大学是共同通讯作者单位。

论文《Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid》于5月21日在线发表。聚二甲硅氧烷材料(PDMS)在Lab on a Chips、BioMEMS等领域,具有重要的应用价值。研究人员将中国传统草药的提取物绿原酸(CA)修饰在PDMS表面,即改变表/界面的亲/疏水特性、又解决了抑菌消毒的问题。CA-coated PDMS的表面,大肠杆菌生长缓慢、有较高的死亡率;用原子力显微镜(AFM)观察E.Coil形态学表明:细菌的细胞壁刚度降低、杨氏模量由3减少至0.8 MPa。CA的涂覆PDMS表面不仅解决了PDMS表面消毒的问题,而且着眼于中国传统草药在科学研究中的应用。

该研究得到了计算机与控制工程学院方勇纯教授主持的国家自然科学基金委项目科学仪器基本研究重点项目、国家自然科学基金、天津科技研究基金、药物化学生物学国家重点实验室开放基金项目的资助。

原文标题:Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid

原文摘要:Coating of polydimethylsiloxane (PDMS) surface with a traditional Chinese herb extract chlorogenic acid (CA) solves the contemporary problem of sterilization of PDMS surface. The E. coli grows slower and has a higher death rate on the CA-coated PDMS surfaces. A smoother morphology of these E. coli cell wall is observed by atomic force microscopy (AFM). Unlike the reported mechanism, wher CA inhibits bacterial growth by damaging the cell membrane in the bulk solution, we find the CA-coated PDMS surface also decreases the stiffness of the cell wall. A decrease in the Young’s modulus of the cell wall from 3 to 0.8 MPa is reported. Unexpectedly, the CA effect on the swarming ability and the biofilm stability of the bacteria can be still observed, even after they have been removed from the CA environment, indicating a decrease in their resistance to antibiotics for a prolonged time. The CA-coated PDMS surface shows better antibiotic effect against three types of both Gram-positive and Gran-negative bacteria than the gentamicin-coated PDMS surface. Coating of CA on PDMS surface not only solves the problem of sterilization of PDMS surface, but also shines light on the application of Chinese traditional herbs in scientific research.

来源: Scientific Reports 浏览次数:0

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