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Sci Rep:中科院版纳植物园所徐增富课题组揭示赤霉素和细胞分裂素相互作用促进小桐子分枝生长分子机理

摘要 : 2017年9月12日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院西双版纳热带植物园徐增富研究组的一篇研究论文

2017年9月12日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院西双版纳热带植物园徐增富研究组的一篇研究论文,研究结果题为Comparative transcriptome analysis of axillary buds in response to the shoot branching regulators gibberellin A3 and 6-benzyladenine in Jatropha curcas。博士研究生倪军和赵美丽为论文第一作者,徐增富研究员为论文通讯作者。

植物分枝或分蘖的特性决定其株型结构,也与其适应环境能力和种子产量密切相关。植物激素在调控植物分枝生长发育过程中起着关键作用,生长素、细胞分裂素和独角金内酯被认为是3种主要的分枝调控激素,而赤霉素的作用被忽视。

科研人员前期的研究发现,赤霉素可有效促进包括小桐子在内的多种木本植物的分枝生长发育。赤霉素促进小桐子分枝生长发育的分子机理,及其在调控分枝生长发育过程中,与细胞分裂素和独角金内酯等其它激素相互作用机制有待深入研究。

研究组采用比较转录组方法,对赤霉素和细胞分裂素相互作用促进小桐子分枝生长的分子机理进行研究。通过对赤霉素(GA3) 和 (BA) 处理的腋芽转录组比较分析,结果显示,有250个基因受到GA3和BA共同调控。其中,共同下调的基因中有一些NAC家族的转录因子,包括JcNAC1,JcNAC2,JcNAC3,JcNAC5,JcNAP,JcNAP-like以及JcNAC047;qPCR分析结果显示,这些NAC基因的表达受到独角金内酯(GR24) 处理的上调,推测他们可能参与分枝生长的调控。此外,在GA3和BA处理后,细胞周期基因CDC6,CDC45和GRF5的表达上调,表明GA3和BA可以通过调节细胞周期机制促进腋芽的生长。更有趣的研究结果是,在腋芽中,BA处理显著上调GA生物合成基因JcGA20oxs和JcGA3ox1的表达,下调GA降解基因JcGA2oxs的表达,这说明细胞分裂素促进分枝,可能部分是通过提高GA的含量来实现的。该比较转录组学研究结果,为进一步阐明植物激素相互作用调控木本植物分枝生长发育的分子机理奠定了基础。

GA3和BA处理三周龄小桐子幼苗促进腋芽生长

原文链接:

Comparative transcriptome analysis of axillary buds in response to the shoot branching regulators gibberellin A3 and 6-benzyladenine in Jatropha curcas

原文摘要:

Cytokinin (CK) is the primary hormone that positively regulates axillary bud outgrowth. However, in many woody plants, such as Jatropha curcas, gibberellin (GA) also promotes shoot branching. The molecular mechanisms underlying GA and CK interaction in the regulation of bud outgrowth in Jatropha remain unclear. To determine how young axillary buds respond to GA3 and 6-benzyladenine (BA), we performed a comparative transcriptome analysis of the young axillary buds ofJatropha seedlings treated with GA3 or BA. Two hundred and fifty genes were identified to be co-regulated in response to GA3 or BA. Seven NACfamily members were down-regulated after treatment with both GA3and BA, wheras these genes were up-regulated after treatment with the shoot branching inhibitor strigolactone. The expressions of the cell cycle genes CDC6, CDC45 and GRF5 were up-regulated after treatment with both GA3 and BA, suggesting they may promote bud outgrowth via regulation of the cell cycle machinery. In the axillary buds, BA significantly increased the expression of GA biosynthesis genesJcGA20oxs and JcGA3ox1, and down-regulated the expression of GA degradation genes JcGA2oxs. Overall, the comprehensive transcriptome data set provides novel insight into the responses of young axillary buds to GA and CK.

来源: Scientific Reports 浏览次数:0

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