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Nature:厦大生科院夏宁邵教授团队合作研制出MERS诊断试剂

摘要 : 最近,韩国发生的中东呼吸综合征(MERS)疫情引起了国内外广泛关注。厦门大学生命科学学院夏宁邵教授团队与香港大学合作成功研制出一种可有效检测中东呼吸综合征冠状病毒(MERS-CoV)的免疫诊断试剂,并于4月22日在Nature出版集团学术刊物《新发微生物和感染》(Emerging Microbes & Infections)在线发表。6月17日,Nature主页(www.nature.com)在“最新研究”(Latest research)栏目头条对该文进行了链接。

 最近,韩国发生的中东呼吸综合征(MERS)疫情引起了国内外广泛关注。厦门大学生命科学学院夏宁邵教授团队与香港大学合作成功研制出一种可有效检测中东呼吸综合征冠状病毒(MERS-CoV)的免疫诊断试剂,并于4月22日在Nature出版集团学术刊物《新发微生物和感染》(Emerging Microbes & Infections)在线发表。6月17日,Nature主页(www.nature.com)在“最新研究”(Latest research)栏目头条对该文进行了链接。

初步研究结果显示其对MERS-CoV具有高灵敏度和高特异性,有望在MERS疫情防控中发挥重要作用。目前,该试剂已做好规模化生产的准备工作,可根据需要随时提供批量试剂。

中东呼吸综合征冠状病毒是2012年发现的新型病毒,被认为和造成SARS的病毒相似。WHO公布的数据显示,截至5月25日全球累计实验室确诊的感染MERS-CoV病例共1139例,其中431例死亡,死亡率37.8%。目前,韩国暴发MERS疫情。截至6月17日,韩国MERS死亡人数增至20人,确诊患者增至162人。

原文标题:A sensitive and specific antigen detection assay for Middle East respiratory syndrome coronavirus

原文摘要:Since its emergence in 2012, more than 900 laboratory-confirmed cases of Middle East respiratory syndrome (MERS) have been reported with a fatality rate of more than 30%. However, no antigen detection assay for commercial use is available for diagnosis. In this study, the full-length nucleocapsid protein (NP) gene of MERS coronavirus (MERS-CoV) was cloned and expressed in Escherichia coli. A MERS-CoV NP capture enzyme-linked immunosorbent assay (ELISA) using two MERS-CoV-NP-specific monoclonal antibodies (MAbs) generated was developed. The ELISA was evaluated using 129 nasopharyngeal aspirates (NPAs) positive for various respiratory viruses and simulated positive NPAs by adding serial dilutions of MERS-CoV. Using a cutoff OD of 0.19, all 129 NPAs positive for respiratory viruses showed very low OD, with a specificity of 100%. For the two simulated MERS-CoV-positive NPAs with serial dilutions of live MERS-CoV, all samples with ≥10 50% tissue culture infective dose (TCID50)/0.1 mL showed positive results. For the 10 additional NPAs with 20 and 200 TCID50/0.1 mL of live MERS-CoV added, all were positive. A highly sensitive and specific MAbs-based antigen capture ELISA has been developed for MERS. This sensitive and specific antigen capture ELISA should be useful for detection of MERS-CoV in human and dromedaries and in field studies.

来源: Emerging Microbes & Infections 浏览次数:0

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