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Nat Commun:同济大学俞作仁研究组揭示抑制miRNA的拉链结构

摘要 : 2017年1月3日,国际学术权威刊物自然出版集团旗下子刊《Nature Communications》杂志在线发表了同济大学东方转化医学研究中心俞作仁教授研究组的一篇研究论文,研究报告了研究人员开发出了另一种miRNAs抑制剂,称为“小RNA拉链”。

2017年1月3日,国际学术权威刊物自然出版集团旗下子刊《Nature Communications》杂志在线发表了同济大学东方转化医学研究中心俞作仁教授研究组的一篇研究论文,研究报告了研究人员开发出了另一种miRNAs抑制剂,称为“小RNA拉链”。这种分子能够将miRNA分子首尾相连,通过一种互补的相互作用,以高度的亲和力、特异性和稳定性,形成一条DNA–RNA双链。

研究人员在人类乳腺癌细胞株中,检测到了两个miRNAs——miR-221和miR-17,表明通过30–50 nM的“小RNA拉链”可以实现70%到90%的miRNA敲除水平。在乳腺癌细胞中,miR-221拉链显示出恢复miR-221靶基因表达以及逆转miR-221致癌功能的能力。此外,这项研究表明,在人乳腺癌细胞中,miR-221拉链可减轻阿霉素耐药性,而且效率比anti-miR-221更高。总之,“小RNA拉链”是一种miRNA抑制剂,可被用来诱导miRNA功能缺失表型,并验证miRNA的靶基因。

原文链接:

Small RNA zippers lock miRNA molecules and block miRNA function in mammalian cells

原文摘要:

MicroRNAs (miRNAs) are a class of singled-stranded small RNA molecules that regulate the stability or translational efficiency of targeted messenger RNAs1. miRNAs are initially transcribed to primary miRNA, which are then cleaved by endonucleases to generate hairpin-structured precursor miRNAs (pre-miRNAs) with 60∼70 nucleotides in length. After transporting to the cytoplasm by Exportin-5, pre-miRNAs are processed by Dicer to generate mature miRNAs with 18∼24 nt in length2,3. More than 2,000 miRNA sequences have been identified or predicted from human origin tissues or cells4. miRNAs are mediated by RNA-induced silencing complex that lead to base-pairing interactions between an miRNA and the binding site of its target mRNAs mostly within the 3′-untranslated region (3′-UTR). miRNAs regulate diverse biological processes including cell fate determination, cell cycle progression, stem cell self-renewal, cancer initiation, progression and metastasis.

来源: Nature Communications 浏览次数:0

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