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Sci Rep:农科院上海兽医所马志永研究组揭示p53基因的抵抗细菌感染作用

摘要 : 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国农业科学院上海兽医研究所马志永研究组题为“Tumor suppressor p53 protects mice against Listeria monocytogenes infection”的研究论文

 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国农业科学院上海兽医研究所马志永研究组题为“Tumor suppressor p53 protects mice against Listeria monocytogenes infection”的研究论文,研究发现肿瘤抑制因子p53具有抵抗单增李斯特菌感染作用,并阐明了其调控宿主免疫反应帮助宿主逃避细菌感染的作用机制。上海兽医所猪呼吸道传染病创新团队首席专家马志永研究员为论文通讯作者,王少辉副研究员为论文第一作者。

研究通过比较单增李斯特菌对p53基因敲除小鼠和正常小鼠的致病性,发现p53基因敲除小鼠的死亡率和病理变化程度均显著高于正常对照组小鼠。细胞感染试验进一步证实了p53可以抑制单增李斯特菌在细胞内的感染及增殖。深入研究发现,p53可以调控宿主的炎症反应及抗细菌感染相关因子,从而发挥抗单增李斯特菌感染的作用。

肿瘤抑制因子p53参与机体的先天性免疫和获得性免疫应答,从而发挥抗病毒感染作用。此项研究成果阐明p53通过转录调控宿主的先天性免疫反应发挥抗单增李斯特菌感染的作用机制,开辟了p53抗细菌感染研究新领域,这必将增加对p53功能的认知,并对细菌病的防控具有重要意义。

原文链接:

Tumor suppressor p53 protects mice against Listeria monocytogenes infection

原文摘要:

Tumor suppressor p53 is involved in regulating immune responses, which contribute to antitumor and antiviral activity. However, whether p53 has anti-bacterial functions remains unclear. Listeria monocytogenes(LM) causes listeriosis in humans and animals, and it is a powerful model for studying innate and adaptive immunity. In the present study, we illustrate an important regulatory role of p53 during LM infection. p53 knockout (p53KO) mice were more susceptible to LM infection, which was manifested by a shorter survival time and lower survival rate. p53KO mice showed significant impairments in LM eradication. Knockdown of p53 in RAW264.7 and HeLa cells resulted in increased invasion and intracellular survival of LM. Furthermore, the invasion and intracellular survival of LM was inhibited in p53-overexpressing RAW264.7 and HeLa cells. LM-infected p53KO mice exhibited severe clinical symptoms and organ injury, presumably because of the abnormal production of the pro-inflammatory cytokines TNF-α, IL-6, IL-12, and IL-18. Decreased IFN-γ and GBP1 productions were observed in LM-infected p53-deficient mice or cells. The combination of these defects likely resulted in the overwhelming LM infection in the p53KO mice. These observations indicate that p53 serves as an important regulator of the host innate immune that protects against LM infection.

来源: Scientific Reports 浏览次数:0

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