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Cell Res:华南农大刘耀光研究组揭示水稻细胞质雄性不育基因起源进化

摘要 : 2016年10月11日,国际学术权威刊物自然出版集团旗下子刊《Cell Research》杂志在线发表了华南农业大学生命科学学院刘耀光研究组题为 "Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes(植物线粒体新细胞质雄性不育基因的形成及演化)的研究论文。

 2016年10月11日,国际学术权威刊物自然出版集团旗下子刊《Cell Research》杂志在线发表了华南农业大学生命科学学院刘耀光研究组题为 "Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes(植物线粒体新细胞质雄性不育基因的形成及演化)的研究论文。论文第一作者为唐辉武博士(博士后),通讯作者是刘耀光研究员和郭晶心副研究员。

新基因起源演化是物种分化和生物多样性的关键因素。在前期研究中,刘耀光团队克隆了在杂交稻广泛利用的野败型细胞质雄性不育(Cytop1asmic Male Sterility,CMS)基因WA352,发现该基因是由多个功能未知的线粒体基因片段重组而成,并揭示出WA352通过与细胞核编码的线粒体蛋白COX11互作导致花粉败育的分子机制(Luo et al.,Nature Genetics,2013)。为了阐明植物线粒体新基因的起源进化机制,该研究对800多份野生稻和栽培稻材料的线粒体基因组进行序列分析,发现了11种与WA352相关的重组结构。通过对这些重组结构的序列相似性比较,转基因功能分析以及回交转育验证,以及蛋白互作分析,重构了这些重组结构复杂的起源进化路径,发现了3个新的CMS基因WA352a,WA352b和WA314。证明这些CMS新基因是由2个结构相似但没有CMS功能的原基因(protogene)通过序列变异和自然选择,获得了与COX11的互作能力即功能化演化而来。

该研究首次提出了植物线粒体基因组的“动态重组--原基因形成--序列和拷贝数变异--功能基因生成”的新基因起源进化模型。该成果将有助于进一步了解CMS基因和恢复基因协同进化和互作的分子机理;同时,该研究成果为植物线粒体新基因的起源和功能化研究提供理论依据,这些新CMS基因的发现将大大扩展杂交稻育种的种质资源。

原文链接:

Multi-step formation, evolution, and functionalization of new cytoplasmic male sterility genes in the plant mitochondrial genomes

原文摘要:

New gene origination is a major source of genomic innovations that confer phenotypic changes and biological diversity. Generation of new mitochondrial genes in plants may cause cytoplasmic male sterility (CMS), which can promote outcrossing and increase fitness. However, how mitochondrial genes originate and evolve in structure and function remains unclear. The rice Wild Abortive type of CMS is conferred by the mitochondrial gene WA352c (previously named WA352) and has been widely exploited in hybrid rice breeding. Here, we reconstruct the evolutionary trajectory of WA352c by the identification and analyses of 11 mitochondrial genomic recombinant structures related to WA352c in wild and cultivated rice. We deduce that these structures arose through multiple rearrangements among conserved mitochondrial sequences in the mitochondrial genome of the wild rice Oryza rufipogon, coupled with substoichiometric shifting and sequence variation. We identify two expressed but nonfunctional protogenes among these structures, and show that they could evolve into functional CMS genes via sequence variations that could relieve the self-inhibitory potential of the proteins. These sequence changes would endow the proteins the ability to interact with the nucleus-encoded mitochondrial protein COX11, resulting in premature programmed cell death in the anther tapetum and male sterility. Furthermore, we show that the sequences that encode the COX11-interaction domains in these WA352c-related genes have experienced purifying selection during evolution. We propose a model for the formation and evolution of new CMS genes via a “multi-recombination/protogene formation/functionalization” mechanism involving gradual variations in the structure, sequence, copy number, and function.

来源: Cell Research 浏览次数:0

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