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Sci Rep:武汉大学丁毅教授研究组发表莲线粒体基因组研究成果

摘要 : 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了武汉大学生命科学学院杂交水稻国家重点实验室丁毅教授团队题为 “The mitochondrial genome map of Nelumbo nucifera reveals ancient evolutionary features” 的研究论文。

 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了武汉大学生命科学学院杂交水稻国家重点实验室丁毅教授团队题为 “The mitochondrial genome map of Nelumbo nucifera reveals ancient evolutionary features” 的研究论文。该论文首次完成了莲线粒体基因组的全基因组测序和线粒体基因组物理图的组装,并揭示了莲作为基部双子叶植物的古老进化特征。该论文共同第一作者为博士生桂松涛和博士吴智华,通讯作者为丁毅教授。

作为孓遗植物的代表之一,对莲的基因组学的研究对于探索高等被子植物进化具有十分重要的意义。在该项研究中,作者利用第三代基因组单分子测序技术(SMRT)对莲的线粒体基因组进行了测序、组装和注释,得到了莲线粒体基因组序列和完整基因组图谱,并揭示了莲线粒体基因组具有进化上的保守性特征,即保留着古老的基因簇和基因数目、高频率的RNA编辑以及低频率的叶绿体片段的插入等现象。这是丁毅教授课题组对莲基因组学研究的系列工作之一,作为国家自然科学基金资助的莲基因组研究计划的一部分工作,莲线粒体基因组图谱的完成,为全面理解莲基因组特征以及探索被子植物进化途径提供了新的线索,也丰富了被子植物基因组进化的数据资料。

研究组今年成果:2013年参与完成了莲( Nelumbo nucifera Gaertn.)核基因组测定(The Plant Journal 2013,76:557-567)、2014年完成莲叶绿体基因组测定(BMC plant biology,2014, 14:289)、2016年5月完成了莲着丝粒蛋白和着丝粒DNA序列的特征研究(The Plant Journal)。

原文链接:

The mitochondrial genome map ofNelumbo nucifera reveals ancient evolutionary features

原文摘要:

Nelumbo nucifera is an evolutionary relic from the Late Cretaceous period. Sequencing the N. nucifera mitochondrial genome is important for elucidating the evolutionary characteristics of basal eudicots. Here, the N. nucifera mitochondrial genome was sequenced using single molecule real-time sequencing technology (SMRT), and the mitochondrial genome map was constructed after de novo assembly and annotation. The results showed that the 524,797-bp N. nuciferamitochondrial genome has a total of 63 genes, including 40 protein-coding genes, three rRNA genes and 20 tRNA genes. Fifteen collinear gene clusters were conserved across different plant species. Approximately 700 RNA editing sites in the protein-coding genes were identified. Positively seleced genes were identified with selection pressure analysis. Nineteen chloroplast-derived fragments were identified, and seven tRNAs were derived from the chloroplast. These results suggest that the N. nucifera mitochondrial genome retains evolutionarily conserved characteristics, including ancient gene content and gene clusters, high levels of RNA editing, and low levels of chloroplast-derived fragment insertions. As the first publicly available basal eudicot mitochondrial genome, the N. nucifera mitochondrial genome facilitates further analysis of the characteristics of basal eudicots and provides clues of the evolutionary trajectory from basal angiosperms to advanced eudicots.

来源: Scientific Reports 浏览次数:0

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