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Sci Rep:农科院杜立新研究团队发现藏羊适应性分子机制

摘要 : 2016年5月27日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表了中国农业科学院北京畜牧兽医研究所杜立新教授研究团队题为“Genome-wide analysis reveals adaptation to high altitudes in Tibetan sheep”的研究成果

 2016年5月27日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表了中国农业科学院北京畜牧兽医研究所杜立新教授研究团队题为“Genome-wide analysis reveals adaptation to high altitudes in Tibetan sheep”的研究成果,论文报道研究人员选择分布于不同海拔高度的7个地方绵羊品种122只绵羊个体进行全基因组扫描,揭示了藏羊高原适应性分子机制。

藏羊分布于青藏高原的地方绵羊品种,经过长期的自然和人工选择对青藏高原特殊环境表现出很强的适应性。研究发现了适应高原恶劣气候环境的主要候选基因有EPAS1,CRYAA,LONP1,NF1,DPP4,SOD1,PPARG和SOCS2等。这些基因主要富集于能量代谢和氧传输、响应缺氧、DNA修复酶等一些适应性性基因和信号通路。其中EPAS1基因在高海拔低氧调控网络中起着核心作用。研究还发现EPAS1基因外显子有12个突变,并在3′UTR区的一个SNP,藏羊CC基因型红细胞计数显著高于TT基因型,而平均红细胞体积和红细胞平均血红蛋白显著低于TT基因型。测量了六个血液学参数在高海拔的品种藏羊和低海拔绵羊品种大尾寒羊。高海拔的品种红血细胞,血红蛋白,红细胞压积,平均红细胞体积和红细胞平均血红蛋白均高于或显著高于低海拔的品种。该研究在基因组水平对藏羊进行了初步的探讨,揭示了藏羊高原适应性。

相关阅读:Sci.Rep:农科院牧医所杜立新研究组发现绵羊体高和体斜长关键基因

原文链接:

Genome-wide analysis reveals adaptation to high altitudes in Tibetan sheep

原文摘要:

Tibetan sheep have lived on the Tibetan Plateau for thousands of years; however, the process and consequences of adaptation to this extreme environment have not been elucidated for important livestock such as sheep. Here, seven sheep breeds, representing both highland and lowland breeds from different areas of China, were genotyped for a genome-wide collection of single-nucleotide polymorphisms (SNPs). The FST and XP-EHH approaches were used to identify regions harbouring local positive selection between these highland and lowland breeds, and 236 genes were identified. We detected selection events spanning genes involved in angiogenesis, energy production and erythropoiesis. In particular, several candidate genes were associated with high-altitude hypoxia, including EPAS1, CRYAA, LONP1, NF1, DPP4, SOD1, PPARG and SOCS2. EPAS1 plays a crucial role in hypoxia adaption; therefore, we investigated the exon sequences of EPAS1 and identified 12 mutations. Analysis of the relationship between blood-related phenotypes and EPAS1 genotypes in additional highland sheep revealed that a homozygous mutation at a relatively conserved site in the EPAS1 3′ untranslated region was associated with increased mean corpuscular haemoglobin concentration and mean corpuscular volume. Taken together, our results provide evidence of the genetic diversity of highland sheep and indicate potential high-altitude hypoxia adaptation mechanisms, including the role of EPAS1 in adaptation.

来源: Scientific Reports 浏览次数:0

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