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Sci Rep:中科院昆明植物所吴建强研究组发表大豆CDPK基因家族响应虫害和干旱研究进展

摘要 : 2016年3月29日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表了中国科学院昆明植物研究所吴建强研究组在大豆CDPK基因家族响应虫害和干旱研究进展

 2016年3月29日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表了中国科学院昆明植物研究所吴建强研究组在大豆CDPK基因家族响应虫害和干旱研究进展,研究题为Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress,孙桂玲研究员和吴建强研究员为论文共同通讯作者。

大豆作为重要的农作物,在农业生产上具有重要地位。每年虫害和干旱等都对大豆生产造成极大的损失。钙离子是普遍存在的第二信使,在真核生物中能够响应发育和胁迫信号并激活一系列的细胞过程。钙离子依赖的蛋白激酶(CDPK)是植物中特有的基因家族,可以感受细胞内外瞬时的钙离子浓度改变而产生活性的变化,并与下游的靶蛋白相互作用,从而将钙离子信息传递下去并引起一系列的生理响应。因此,是植物中较重要的一类信号传导因子。

为了解析大豆CDPK基因家族各成员在虫害和干旱胁迫下的不同功能,吴建强研究组首先对大豆基因组中所有的CDPK成员进行了生物信息学基因鉴定,发现大豆基因组中共有50个CDPK基因家族的成员;系统发育分析表明这50个家族成员分为13个亚类,并且这13个亚类在单子叶和双子叶分化之前就已经形成了。基因表达分析显示这些家族成员具有明显的组织表达特异性。各家族成员对咀嚼式和刺吸式昆虫具有特定的响应模式,并且不受茉莉酸和水杨酸的调控。在干旱和ABA的处理下,一多半的家族成员的转录水平都发生了显著变化,表明CDPK在植物抗非生物胁迫中发挥着重要角色。该研究为了解大豆的抗性生理和培育抗虫、抗旱新品种提供了较重要的数据。

大豆、百脉根、苜蓿、拟南芥和水稻CDPK的系统发育分析。各物种中CDPK的基因数目为:大豆,50个;百脉根,19个;苜蓿,25个;拟南芥个,34;水稻,29个。

原文链接:

Genome-wide identification of calcium-dependent protein kinases in soybean and analyses of their transcriptional responses to insect herbivory and drought stress

原文摘要:

Calcium-dependent protein kinases (CDPKs) are plant-specific calcium sensors that play important roles in various aspects of plant physiology. Here, we investigated phylogenic relationships, chromosomal locations, gene structures, and tissue-specific, herbivory- and drought-induced expression profiles of soybean (Glycine max)GmCDPKs. Fifty GmCDPK genes were identified, which phylogenetically grouped into 4 distinct clusters and distributed across 13 sub-clusters. Individual classes ofGmCDPKs harbor highly conserved mRNA splicing sites, and their exon numbers and lengths were consistent with the phylogenetic relationships, suggesting that at least 13 ancestral CDPK genes had emerged before the split of monocots and eudicots. Gene expression analysis indicated that several GmCDPKs were tissue-specific expressed.GmCDPKs’ transcript levels changed after wounding, exhibited specific expression patterns after simulated Spodoptera exigua feeding or soybean aphid (Aphis glycines) herbivory, and were largely independent of the phytohormones jasmonic acid and salicylic acid. The most pronounced transcriptional responses were detected after drought and abscisic acid treatments with more than half of all GmCDPKs being upregulated, suggesting their important roles during abiotic stress responses in soybean. Our data provide an important foundation for further functional dissection of GmCDPKs, especially in the context of soybean-insect interactions and drought stress adaptation.

来源: Scientific Reports 浏览次数:0

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