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Nature:由RNA控制的DNA修复

标签:DNA RNA
摘要 : Francesca Storici及同事现在发现,内源RNA转录体能介导与酵母染色体DNA的重组。这一结果说明,遗传信息在细胞中从RNA向DNA的直接流动也许要比我们过去所认为的普遍得多,而且因为细胞核中的RNA水平非常高,所以这些结果也许会让我们对修复的可塑性和基因组不稳定性的机制有新的认识。

nature13682-f1

 如其名称所示,通过同源重组进行的DNA修复一般被认为发生在两个DNA分子之间。然而,研究工作已经证明,RNA也可用在人造环境中。

Francesca Storici及同事现在发现,内源RNA转录体能介导与酵母染色体DNA的重组。这一结果说明,遗传信息在细胞中从RNA向DNA的直接流动也许要比我们过去所认为的普遍得多,而且因为细胞核中的RNA水平非常高,所以这些结果也许会让我们对修复的可塑性和基因组不稳定性的机制有新的认识。

原文摘要:

Transcript-RNA-templated DNA recombination and repair

Havva Keskin, Ying Shen, Fei Huang, Mikir Patel, Taehwan Yang, Katie Ashley, Alexander V. Mazin & Francesca Storici

Homologous recombination is a molecular process that has multiple important roles in DNA metabolism, both for DNA repair and genetic variation in all forms of life1. Generally, homologous recombination involves the exchange of genetic information between two identical or nearly identical DNA molecules1; however, homologous recombination can also occur between RNA molecules, as shown for RNA viruses2. Previous research showed that synthetic RNA oligonucleotides can act as templates for DNA double-strand break (DSB) repair in yeast and human cells3, 4, and artificial long RNA templates injected in ciliate cells can guide genomic rearrangements5. Here we report that endogenous transcript RNA mediates homologous recombination with chromosomal DNA in yeastSaccharomyces cerevisiae. We developed a system to detect the events of homologous recombination initiated by transcript RNA following the repair of a chromosomal DSB occurring either in a homologous but remote locus, or in the same transcript-generating locus in reverse-transcription-defective yeast strains. We found that RNA–DNA recombination is blocked by ribonucleases H1 and H2. In the presence of H-type ribonucleases, DSB repair proceeds through a complementary DNA intermediate, wheras in their absence, it proceeds directly through RNA. The proximity of the transcript to its chromosomal DNA partner in the same locus facilitates Rad52-driven homologous recombination during DSB repair. We demonstrate that yeast and human Rad52 proteins efficiently catalyse annealing of RNA to a DSB-like DNA end in vitro. Our results reveal a novel mechanism of homologous recombination and DNA repair in which transcript RNA is used as a template for DSB repair. Thus, considering the abundance of RNA transcripts in cells, RNA may have a marked impact on genomic stability and plasticity.(doi: 10.1038/nature13682

对应Nature杂志: 2014年11月20日Nature杂志精选

来源: Nature中文 浏览次数:132

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