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食道癌的基因组分析

摘要 : 中国协和医科大学等处的研究人员在新研究中对来自超过100例食道鳞状细胞癌的样本所做的基因组分析,识别出8个显著突变的基因,其中6个众所周知是与肿瘤相关的基因,两个(ADAM29 和FAM135B)以前没有在这种类型的癌症中被描述过。相关文章发表于2014年3月16日的《Nature》杂志上。

这篇论文报告了对来自超过100例食道鳞状细胞癌的样本所做的基因组分析。

研究人员识别出了8个显著突变的基因,其中6个众所周知是与肿瘤相关的基因,两个(ADAM29 和FAM135B)以前没有在这种类型的癌症中被描述过。分析显示了几个重要组蛋白调控基因的频发突变,识别出在放大的11q13.3-13.4区域被编码的一个微RNA为一个新的致癌基因。

而且,食道癌还被发现在遗传上与头部和颈部鳞状细胞癌有共同致病机制。

原文摘要:

Identification of genomic alterations in oesophageal squamous cell cancer

Yongmei Song, Lin Li, Yunwei Ou, Zhibo Gao, Enmin Li, Xiangchun Li, Weimin Zhang,Jiaqian Wang, Liyan Xu, Yong Zhou, Xiaojuan Ma, Lingyan Liu, Zitong Zhao, Xuanlin Huang,Jing Fan, Lijia Dong, Gang Chen, Liying Ma, Jie Yang, Longyun Chen, Minghui He, Miao Li,Xuehan Zhuang, Kai Huang, Kunlong Qiu et al.

Oesophageal cancer is one of the most aggressive cancers and is the sixth leading cause of cancer death worldwide. Approximately 70% of global oesophageal cancer cases occur in China, with oesophageal squamous cell carcinoma (ESCC) being the histopathological form in the vast majority of cases (>90%). Currently, there are limited clinical approaches for the early diagnosis and treatment of ESCC, resulting in a 10% five-year survival rate for patients. However, the full repertoire of genomic events leading to the pathogenesis of ESCC remains unclear. Here we describe a comprehensive genomic analysis of 158 ESCC cases, as part of the International Cancer Genome Consortium research project. We conducted whole-genome sequencing in 17 ESCC cases and whole-exome sequencing in 71 cases, of which 53 cases, plus an additional 70 ESCC cases not used in the whole-genome and whole-exome sequencing, were subjected to array comparative genomic hybridization analysis. We identified eight significantly mutated genes, of which six are well known tumour-associated genes (TP53, RB1, CDKN2A, PIK3CA, NOTCH1,NFE2L2), and two have not previously been described in ESCC (ADAM29 and FAM135B). Notably,FAM135B is identified as a novel cancer-implicated gene as assayed for its ability to promote malignancy of ESCC cells. Additionally, MIR548K, a microRNA encoded in the amplified 11q13.3-13.4 region, is characterized as a novel oncogene, and functional assays demonstrate thatMIR548K enhances malignant phenotypes of ESCC cells. Moreover, we have found that several imp0rtant histone regulator genes (MLL2 (also called KMT2D), ASH1L, MLL3 (KMT2C), SETD1B,CREBBP and EP300) are frequently altered in ESCC. Pathway assessment reveals that somatic aberrations are mainly involved in the Wnt, cell cycle and Notch pathways. Genomic analyses suggest that ESCC and head and neck squamous cell carcinoma share some common pathogenic mechanisms, and ESCC development is associated with alcohol drinking. This study has explored novel biological markers and tumorigenic pathways that would greatly improve therapeutic strategies for ESCC.

对应Nature杂志: 2014年5月1日的Nature杂志精选

来源: Nature中文 浏览次数:111

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