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Nat Commun:华中农大罗杰研究组发布水稻代谢组研究成果

摘要 : 2016年10月4日,国际学术权威刊物自然出版集团旗下子刊《Nature Communications》杂志在线发表了华中农业大学生命科学技术学院作物遗传改良国家重点实验室罗杰教授研究组题为“Comparative and parallel genome-wide association studies for metabolic and agronomic traits in cereals”的研究论文。

 2016年10月4日,国际学术权威刊物自然出版集团旗下子刊《Nature Communications》杂志在线发表了华中农业大学生命科学技术学院作物遗传改良国家重点实验室罗杰教授研究组题为“Comparative and parallel genome-wide association studies for metabolic and agronomic traits in cereals”的研究论文。陈伟博士及博士研究生王文升、彭梦为论文共同第一作者,罗杰教授为论文通讯作者。

论文在前期水稻叶片(Chen et al., Nat Genet, 2014, 46:14-721)及玉米籽粒(Wen et al., Nat Commun, 2014, 5:3438)代谢组研究基础上,对水稻种子代谢及农艺性状的遗传基础开展研究。论文首先揭示了水稻代谢物的遗传调控存在明显的组织特异性,且这种特异性部分受到组织特异性表达的等位基因控制。在此基础上,利用水稻和玉米在遗传定位效力及定位精度方面互补的特点,通过比较全基因组关联分析,同时提高了基因定位的效力和定位精度,确定了近20个新的控制水稻种子代谢物含量的候选基因,并对其中部分基因进行了功能验证。另外,通过对水稻种子农艺(颜色、粒重、粒厚、粒长和粒宽)及代谢性状“共调控”的平行全基因组关联分析,克隆并鉴定了部分同时影响代谢及农艺性状的基因。例如,控制葫芦巴碱合成的关键基因氧甲基转移酶(Os02g57760)既控制葫芦巴碱的含量,同时也影响水稻粒宽。上述研究促进了作物代谢物遗传及生化调控的解析,提供了代谢与农艺性状关联的直接证据,同时提供了利用“交互功能基因组及代谢组”进行作物复杂性状解析的新手段。

原文链接:

Comparative and parallel genome-wide association studies for metabolic and agronomic traits in cereals

原文摘要:

The plant metabolome is characterized by extensive diversity and is often regarded as a bridge between genome and phenome. Here we report metabolic and phenotypic genome-wide studies (mGWAS and pGWAS) in rice grain that, in addition to previous metabolic GWAS in rice leaf and maize kernel, show both distinct and overlapping aspects of genetic control of metabolism within and between species. We identify new candidate genes potentially influencing important metabolic and/or morphological traits. We show that the differential genetic architecture of rice metabolism between different tissues is in part determined by tissue specific expression. Using parallel mGWAS and pGWAS we identify new candidate genes potentially responsible for variation in traits such as grain colour and size, and provide evidence of metabotype-phenotype linkage. Our study demonstrates a powerful strategy for interactive functional genomics and metabolomics in plants, especially the cloning of minor QTLs for complex phenotypic traits.

来源: Nature Communications 浏览次数:0

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