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Sci Rep:中科院成都生物所江建平研究组发表两栖动物变态发育研究进展

摘要 : 2016年6月2日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院成都生物研究所江建平研究组题为“Transcriptome profiles of metamorphosis in the ornamented pygmy frog Microhylafissipes clarifythe functions of thyroid hormonereceptors in metamorphosis”的研究论文

 2016年6月2日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院成都生物研究所江建平研究组题为“Transcriptome profiles of metamorphosis in the ornamented pygmy frog Microhylafissipes clarifythe functions of thyroid hormonereceptors in metamorphosis”的研究论文,博士研究生赵兰英同学为论文第一作者,江建平研究员为论文通讯作者。

态发育在两栖动物发育中是普遍现象,“小蝌蚪找妈妈”的故事是变态发育的生动而形象的展示。那么,蝌蚪的变态发育调控机制如何呢。这是一个极其复杂的科学问题。

早期研究已经证明甲状腺素在调控无尾两栖动物变态发育中起到至关重要的作用。已有利用模式生物爪蟾Xenopus laevis研究发现有两种甲状腺素受体α(Thyriod Hormone α,TRα)和β(Thyriod Hormone β,TRβ)介导甲状腺素的调控作用。已有研究报道爪蟾TRα敲低或敲除时,爪蟾蝌蚪发育加快但变态个体明显变小,而TRα的这些功能在其它物种中是否保守还不清楚;同时过去对于变态发育调控分子机制的推论中,TRα作为转录因子开启变态发育的发生,但是敲除TRα的个体依然能够进行变态发育,这暗示了在变态发育中存在其它的转录因子调控变态发育。

研究人员以其正在研发的新型模式生物饰纹姬蛙Microhyla fissipes为研究对象,检测了3个关键发育时期变态前期(premetamorphosis)、变态高峰期(metamorphic climax)和变态完成期(completion of metamorphosis)的转录组,共获得2293基因表现出发育时期的差异表达,其中非常有趣的发现是在变态前期时,TRα基因在体型明显较大并且到达变态发育期时间较长的物种(非洲爪蟾和中华蟾蜍Bufogargarizans)中高表达,而在体型明显小且发育时间较短的饰纹姬蛙中低表达;同时我们也发现TRβ无论在饰纹姬蛙和非洲爪蟾,甚至在敲除了TRα的爪蟾个体中均高表达,这暗示了TRβ是开启变态发育发生的关键转录因子。因此,本项工作不仅鉴定出可能与变态发育有关的多个基因,更重要的是揭示。去结合甲状腺激素的TRα调控蝌蚪生长和变态时间的作用可能在无尾两栖动物中是保守的,而结合甲状腺激素的TRβ是开启变态发育所必需的转录因子。

原文链接:

Transcriptome profiles of metamorphosis in the ornamented pygmy frog Microhylafissipes clarifythe functions of thyroid hormonereceptors in metamorphosis

原文摘要:

Anuran metamorphosis is an excellent system in which to study postembryonic development. Studies on Xenopus (Mesobatrachia) show that thyroid hormone receptors (TRs) regulate metamorphosis in a ligand-dependent manner by coordinating the action of hundreds of genes. However, whether this mechanism is conserved among amphibians is still unknown. To understand the molecular mechanism of this universal phenomenon, we report the transcriptional profiles of the three key developmental stages in Microhyla fissipes (Neobatrachia): premetamorphosis (PM), metamorphic climax (MC) and completion of metamorphosis (CM). In total, 2,293 differentially expressed genes were identified from comparisons of transcriptomes, and these genes showed stage-specific expression patterns. Unexpectedly, we found that TRα was highly expressed in Xenopus laevis and Bufo gargarizans at premetamorphosis but showed low expression in M. fissipes. In contrast, TRβ was highly expressed during metamorphosis in M. fissipes and X. laevis. This result may imply that TRβ is essential for initiating metamorphosis, at least in M. fissipes. Thus, our work not only identifies genes that are likely to be involved in Neobatrachia metamorphosis but also clarifies the roles of unliganded TRα in regulating tadpole growth and timing of metamorphosis, which may be conserved in anurans, and the role of liganded TRβ in launching metamorphosis.

来源: Scientific Reports 浏览次数:0

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