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摘要 : 由瑞士日内瓦大学的医学院的Stylianos Antonarakis领导的一个研究组对在21号染色体的“三体”性上有所不同的单卵双胞胎的胎儿细胞的转录组研究表明,该双胞胎之间的差异化表达沿所有染色体都是按不同区域组织的。相关文章发表于2014年4月16日的《Nature》杂志上。





Domains of genome-wide gene expressi0n dysregulation in Down’s syndrome

Audrey Letourneau, Federico A. Santoni, Ximena Bonilla, M. Reza Sailani, David Gonzalez,Jop Kind, Claire Cheva1ier, Robert Thurman, Richard S. Sandstrom, Youssef Hibaoui,Marco Garieri, Konstantin Popadin, Emilie Falconnet, Maryline Gagnebin, Corinne Gehrig,Anne Vannier, Michel Guipponi, Laurent Farinelli, Daniel Robyr, Eugenia Migliavacca,Christelle Borel, Samuel Deutsch, Anis Feki, John A. Stamatoyannopoulos, Yann Heraultet al.

Trisomy 21 is the most frequent genetic cause of cognitive impairment. To assess the perturbations of gene expressi0n in trisomy 21, and to eliminate the noise of genomic variability, we studied the transcr-ptome of fetal fibroblasts from a pair of monozygotic twins discordant for trisomy 21. Here we show that the differential expressi0n between the twins is organized in domains along all chromosomes that are either upregulated or downregulated. These gene expressi0n dysregulation domains (GEDDs) can be defined by the expressi0n level of their gene content, and are well conserved in induced pluripotent stem cells derived from the twins’ fibroblasts. Comparison of the transcr-ptome of the Ts65Dn mouse model of Down’s syndrome and normal littermate mouse fibroblasts also showed GEDDs along the mouse chromosomes that were syntenic in human. The GEDDs correlate with the lamina-associated (LADs) and replication domains of mammalian cells. The overall position of LADs was not altered in trisomic cells; however, the H3K4me3 profile of the trisomic fibroblasts was modified and accurately followed the GEDD pattern. These results indicate that the nuclear compartments of trisomic cells undergo modifications of the chromatin environment influencing the overall transcr-ptome, and that GEDDs may therefore contribute to some trisomy 21 phenotypes.

对应Nature杂志: 2014年4月17日Nature杂志精选

来源: Nature中文 浏览次数:112


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