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摘要 : 线粒体内膜中的 OXPHOS (氧化磷酸化) 复合物在NADH被分子氧氧化过程中通过ATP从ADP和无机磷酸盐的合成产生细胞的绝大部分能量。

 线粒体内膜中的 OXPHOS (氧化磷酸化) 复合物在NADH被分子氧氧化过程中通过ATP从ADP和无机磷酸盐的合成产生细胞的绝大部分能量。由于OXPOS复合物含有被核基因组和线粒体基因组编码的亚单元,所以人们曾普遍假设,这两个腔室之间一定存在沟通,来协调基因表达。 Stirling Churchman及同事现在表征了OXPHOS亚单元的合成。他们发现,核转录程序和线粒体转录程序是在核基因组引导下被独立调控的。调控不是在转录层面发生的,而是在翻译层面发生的,其中线粒体翻译是通过胞质核糖体被调控的。


Synchronized mitochondrial and cytosolic translation programs


Oxidative phosphorylation (OXPHOS) is a vital process for energy generation, and is carried out by complexes within the mitochondria. OXPHOS complexes pose a unique challenge for cells because their subunits are encoded on both the nuclear and the mitochondrial genomes. Genomic approaches designed to study nuclear/cytosolic and bacterial gene expression have not been broadly applied to mitochondria, so the co-regulation of OXPHOS genes remains largely unexplored. Here we monitor mitochondrial and nuclear gene expression in Saccharomyces cerevisiae during mitochondrial biogenesis, when OXPHOS complexes are synthesized. We show that nuclear- and mitochondrial-encoded OXPHOS transcript levels do not increase concordantly. Instead, mitochondrial and cytosolic translation are rapidly, dynamically and synchronously regulated. Furthermore, cytosolic translation processes control mitochondrial translation unidirectionally. Thus, the nuclear genome coordinates mitochondrial and cytosolic translation to orchestrate the timely synthesis of OXPHOS complexes, representing an unappreciated regulatory layer shaping the mitochondrial proteome. Our whole-cell genomic profiling approach establishes a foundation for studies of global gene regulation in mitochondria.

来源: Nature 浏览次数:0


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