DOI：10.1038/nature17948 作者：Ali Brivanlou
摘要 : Ali Brivanlou及同事建立了一个体外模型，来研究在囊胚形成与宫内移植之间引导人类胚胎发育的事件。
Ali Brivanlou及同事建立了一个体外模型，来研究在囊胚形成与宫内移植之间引导人类胚胎发育的事件。他们描述了在外胚层扩张(epiblast expansion)、世系分离(lineage segregation)、滋养层分化(trophoblast specification)、双层胚盘形成(bi-laminar disc formation)和卵黄囊空化(yolk sac cavitation)期间的关键物种特异性事件。
Implantation of the blastocyst is a developmental milestone in mammalian embryonic development. At this time, a coordinated program of lineage diversification, cell-fate specification, and morphogenetic movements establishes the generation of extra-embryonic tissues and the embryo proper, and determines the conditions for successful pregnancy and gastrulation. Despite its basic and clinical importance, this process remains mysterious in humans. Here we report the use of a novel in vitro system1, 2 to study the post-implantation development of the human embryo. We unveil the self-organizing abilities and autonomy of in vitro attached human embryos. We find human-specific molecular signatures of early cell lineage, timing, and architecture. Embryos display key landmarks of normal development, including epiblast expansion, lineage segregation, bi-laminar disc formation, amniotic and yolk sac cavitation, and trophoblast diversification. Our findings highlight the species-specificity of these developmental events and provide a new understanding of early human embryonic development beyond the blastocyst stage. In addition, our study establishes a new model system relevant to early human pregnancy loss. Finally, our work will also assist in the rational design of differentiation protocols of human embryonic stem cells to specific cell types for disease modelling and cell replacement therapy.
来源： Nature 浏览次数：0