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Sci Rep:浙江大学农学院陈昆松教授揭示调控柑橘柠檬酸积累的新转录因子

摘要 : 2016年3月17日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志上在线发表了浙江大学农学院果实品质生物学陈昆松教授研究团队题为“The Citrus transcription factor, CitERF13, regulates citric acid accumulation via a protein-protein interaction with the vacuolar proton pump, CitVHA-c4”最新研究论文

 2016年3月17日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志上在线发表了浙江大学农学院果实品质生物学陈昆松教授研究团队题为“The Citrus transcription factor, CitERF13, regulates citric acid accumulation via a protein-protein interaction with the vacuolar proton pump, CitVHA-c4”最新研究论文,研究发现柑橘AP2/ERF基因家族成员,CitERF13可与泡膜型质子泵c亚基,CitVHA-c4发生蛋白互作,进而协同调控柠檬酸积累。博士研究生李绍佳和殷学仁副教授为论文共同第一作者,陈昆松教授为论文通讯作者。

有机酸是决定果实风味品质的重要因素之一,柑橘果实的有机酸主要为柠檬酸,柠檬酸含量由合成、降解、运转等多个步骤决定,每一步骤均有多种酶/多基因家族调控。本研究通过对椪柑果实(普通椪柑和早熟椪柑)中编码质子泵基因成员进行分析,认为CitVHA-c4可能促进柠檬酸在液泡中积累,进一步通过酵母双杂交、双分子荧光互补体系发现并验证一个AP2/ERF成员,CitERF13,其可与CitVHA-c4存在蛋白互作。最后,利用普通烟草、拟南芥突变体叶片瞬时表达体系进行功能验证,结果表明,CitERF13可通过与CitVHA-c4蛋白互作,进而促进液泡中柠檬酸的积累。

原文链接:

The Citrus transcription factor, CitERF13, regulates citric acid accumulation via a protein-protein interaction with the vacuolar proton pump, CitVHA-c4

原文摘要:

Organic acids are essential to fruit flavor. The vacuolar H+ transporting adenosine triphosphatase (V-ATPase) plays an important role in organic acid transport and accumulation. However, less is known of V-ATPase interacting proteins and their relationship with organic acid accumulation. The relationship between V-ATPase and citric acid was investigated, using the citrus tangerine varieties ‘Ordinary Ponkan (OPK)’ and an early maturing mutant ‘Zaoshu Ponkan (ZPK)’. Five V-ATPase genes (CitVHA) were predicted as important to citric acid accumulation. Among the genes, CitVHA-c4 was observed, using a yeast two-hybrid screen, to interact at the protein level with an ethylene response factor, CitERF13. This was verified using bimolecular fluorescence complementation assays. A similar interaction was also observed between Arabidopsis AtERF017 (a CitERF13 homolog) and AtVHA-c4 (a CitVHA-c4 homolog). A synergistic effect on citric acid levels was observed between V-ATPase proteins and interacting ERFs when analyzed using transient over-expression in tobacco and Arabidopsis mutants. Furthermore, the transcript abundance of CitERF13 was concomitant with CitVHA-c4. CitERF13 or AtERF017 over-expression leads to significant citric acid accumulation. This accumulation was abolished in an AtVHA-c4 mutant background. ERF-VHA interactions appear to be involved in citric acid accumulation, which was observed in both citrus and Arabidopsis.

来源: Scientific Reports 浏览次数:0

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