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Sci.Rep:中科院成都生物所李大平组在中链脂肪酸生物合成研究中获进展

摘要 : 9月25日,《Nature》杂志子刊《Scientific Report》上发表中国科学院成都生物研究所李大平课题组在链脂肪酸生物合成研究进展,题为“The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production”.

 9月25日,《Nature》杂志子刊《Scientific Report》上发表中国科学院成都生物研究所李大平课题组在链脂肪酸生物合成研究进展,题为“The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production”.

中链脂肪酸(如己酸)是含有6个及以上碳原子的饱和脂肪酸,是生物燃料的合成前体,同时具有重要的化工用途。由于己酸溶解度低,易提取,具有高附加值,国际上现已将其视为一种新兴能源物质。

李大平课题组副研究员陶勇和助理研究员朱晓宇首次发现中链脂肪酸己酸可以由微生物催化乳酸而合成,并由此提出一个新的己酸合成路径:乳酸氧化耦合脂肪酸β氧化逆循环。研究表明,该路径可以实现己酸的高效合成,积累浓度可以超过23 g/L,比国际上现有报道提高了82.89%。己酸合成产率可以达到2.97g/L/d,已具备工业化应用的潜力。目前,己酸的生物合成主要是通过以克氏梭菌(Clostridium kluyveri)为核心的微生物催化乙醇合成。该研究报道的以乳酸为底物合成己酸,是己酸合成理论的重要补充,也是己酸合成技术的重大突破。此外,该研究揭示了浓香型白酒的主体香味物质前体——己酸可能存在新的合成机制。

合成途径

原文链接:The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production

原文摘要:

A unique microbiome that metabolizes lactate rather than ethanol for n-caproate production was obtained from a fermentation pit used for the production of Chinese strong-flavour liquor (CSFL). The microbiome was able to produce n-caproate at concentrations as high as 23.41 g/L at a maximum rate of 2.97 g/L/d in batch trials without in-line extraction. Compared with previous work using ethanol as the electron donor, the n-caproate concentration increased by 82.89%. High-throughput sequencing analysis showed that the microbiome was dominated by aClostridium cluster IV, which accounted for 79.07% of total reads. A new process for n-caproate production was proposed, lactate oxidation coupled to chain elongation, which revealed new insight into the well-studied lactate conversion and carbon chain elongation. In addition, these findings indicated a new synthesis mechanism of n-caproate in CSFL. We believe that this efficient process will provide a promising opportunity for the innovation of waste recovery as well as forn-caproate biosynthesis.

来源: Scientific Reports 浏览次数:0

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