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Nature:逆转录病毒在干细胞多能性中的参与情况

摘要 : 对人“诱导多能干”(iPS)和“胚胎干”(ES)细胞所做的这项广泛分析,识别出一个子类群的细胞,它们显示出灵长类特有的内源性逆转录病毒HERVH的转录水平升高,同时还表现出初始态细胞的特征。

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对人“诱导多能干”(iPS)和“胚胎干”(ES)细胞所做的这项广泛分析,识别出一个子类群的细胞,它们显示出灵长类特有的内源性逆转录病毒HERVH的转录水平升高,同时还表现出初始态细胞的特征。

HERVH和转录因子 LBP9被发现驱动多能细胞特有的转录体的表达,包括调控性的长非编码RNA。

作者提出,这些发现表明,存在着一个以前没有被识别出的、灵长类特有的调控转录回路的多能性。

原文标题:Primate-specific endogenous retrovirus-driven transcription defines naive-like stem cells

原文摘要:Naive embryonic stem cells hold great promise for research and therapeutics as they have broad and robust developmental potential. While such cells are readily derived from mouse blastocysts it has not been possible to isolate human equivalents easily1, 2, although human naive-like cells have been artificially generated (rather than extracted) by coercion of human primed embryonic stem cells by modifying culture conditions2, 3, 4 or through transgenic modification5. Here we show that a sub-population within cultures of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) manifests key properties of naive state cells. These naive-like cells can be genetically tagged, and are associated with elevated transcription of HERVH, a primate-specific endogenous retrovirus. HERVH elements provide functional binding sites for a combination of naive pluripotency transcription factors, including LBP9, recently recognized as relevant to naivety in mice6. LBP9–HERVH drives hESC-specific alternative and chimaeric transcripts, including pluripotency-modulating long non-coding RNAs. Disruption of LBP9, HERVH and HERVH-derived transcripts compromises self-renewal. These observations define HERVH expression as a hallmark of naive-like hESCs, and establish novel primate-specific transcriptional circuitry regulating pluripotency.

对应Nature杂志: 2014年12月18日Nature杂志精选

来源: Nature 浏览次数:270

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