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Sci Rep:中科院上海生科院张鹏研究组发现调控淀粉代谢并影响储藏根产量的关键基因

摘要 : 2017年8月29日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院上海生命科学研究院植物生理生态研究所张鹏研究组的一篇研究论文,研究成果题为Alpha-Glucan, Water Dikinase 1 Affects Starch Metabolism and Storage Root Growth in Cassava (Manihot esculenta Crantz)

2017年8月29日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院上海生命科学研究院植物生理生态研究所张鹏研究组的一篇研究论文,研究成果题为Alpha-Glucan, Water Dikinase 1 Affects Starch metabolism and Storage Root Growth in Cassava (Manihot esculenta Crantz),论文报道了调控淀粉代谢并影响储藏根产量的关键基因。文章第一作者为张鹏研究组博士周文智,张鹏研究员为论文通讯作者。

木薯是全球第三大粮食作物,其储藏根可大量富集淀粉,是热带亚热带地区近7亿人口的主要食物能量来源。研究木薯淀粉代谢的调控对深入了解这一重要粮食作物的源库分配机制及提高产量具有重要的理论价值和应用潜力。

张鹏研究组利用第一个木薯T-DNA插入突变体storage root delay (srd),发现了导致储藏根发育延缓的关键基因是参与淀粉磷酸化的关键基因α-葡聚糖,水合二激酶1 (GWD1)。该基因表达严重缺失可抑制地上叶片临时型淀粉的及时降解,导致叶片大量富集淀粉,改变临时型淀粉粒的形态建成,影响光合作用及从源向库的碳水化合物分配,进而延缓储藏根的生长和发育。研究表明GWD1是通过磷酸化淀粉并与β-amylase共同协作来调控木薯淀粉的降解。该研究为进一步强化临时型淀粉的降解,促进源库分配提供了新思路和技术;同时,为综合利用木薯叶片作为优质饲料提供了新种质。

调控淀粉代谢并影响储藏根产量的关键基因

原文链接:

Alpha-Glucan, Water Dikinase 1 Affects Starch metabolism and Storage Root Growth in Cassava (Manihot esculentaCrantz)

原文摘要:

Regulation of storage root development by source strength remains largely unknown. The cassava storage root delay (srd) T-DNA mutant postpones storage root development but manifests normal foliage growth as wild-type plants. The SRD gene was identified as an orthologue of α-glucan, water dikinase 1 (GWD1), whose expression is regulated under conditions of light/dark cycles in leaves and is associated with storage root development. The GWD1-RNAi cassava plants showed both retarded plant and storage root growth, as a result of starch excess phenotypes with reduced photosynthetic capacity and decreased levels of soluble saccharides in their leaves. These leaves contained starch granules having greatly increased amylose content and type C semi-crystalline structures with increased short chains that suggested storage starch. In storage roots of GWD1-RNAi lines, maltose content was dramatically decreased and starches with much lower phosphorylation levels showed a drastically reduced β-amylolytic rate. These results suggested that GWD1 regulates transient starch morphogenesis and storage root growth by decreasing photo-assimilation partitioning from the source to the sink and by starch mobilization in root crops.

来源: Scientific Reports 浏览次数:0

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