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Sci Rep:中科院华南植物园张新华研究员发表檀香冷响应机制研究进展

摘要 : 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院华南植物园博士张新华的一篇研究论文,研究发表了檀香冷响应机制。

 近日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》杂志在线发表了中国科学院华南植物园博士张新华的一篇研究论文,研究发表了檀香冷响应机制。

植物与环境之间存在着极为密切的联系。任何植物,无论是一个个体,还是群体,都需要随时随地应对所在的环境并做出积极的响应,这是生命维持其存在和发展的必由之路。冷胁迫是阻碍植物生长、限制其地理分布与减少农作物产量的主要的非生物因子之一。檀香(Santalum album L., sandalwood)堪称世界上最贵的木本植物之一,素有“绿色黄金”之称。目前,主要栽培于中国、斯里兰卡、印度尼西亚、马来西亚、菲律宾和澳大利亚北部等东南亚国家。在我国,檀香主要在广东、海南和福建等热带亚热带地区种植。低温是影响檀香生长与分布的主要环境因子之一。但目前有关檀香对低温胁迫的响应与适应机制仍不清楚。

科研人员对低温(4℃)处理条件下檀香冷响应和适应机制进行了较全面的研究。结果显示:在低温胁迫条件下,檀香具有积极的冷响应保护机制。表现在:丙二醛(Malondialdehyde)、脯氨酸(Proline)和可溶性糖(Soluble carbohydrates)的积累,抗氧化物酶SOD与POD的水平显著增加;细胞膜转运、Ca离子、ROS,以及植物激素信号等相关的转录本显著性上调表达;更重要的发现,在冷胁迫中8个檀香萜烯化合酶基因在植物叶与根组织中存在重要的差异表达;另外鉴定了4个冷驯化关键调节基因-CBFs转录因子,其中,在冷胁迫12 h和24 h时,CBF2-CBF4的表达量是对照组的50-200倍,结果暗示了依赖CBF的冷信号转导途径很可能在檀香冷忍耐中扮演关键的角色。该研究结果为檀香栽培与育种,以及檀香油生物合成机制研究提供重要的理论基础。

檀香冷响应机制

原文链接:

Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves

原文摘要:

Santalum album L. (Indian sandalwood) is an economically important plant species because of its ability to produce highly valued perfume oils. Little is known about the mechanisms by which S. album adapts to low temperatures. In this study, we obtained 100,445,724 raw reads by paired-end sequencing from S. album leaves. Physiological and transcriptomic changes in sandalwood seedlings exposed to 4 °C for 0–48 h were characterized. Cold stress induced the accumulation of malondialdehyde, proline and soluble carbohydrates, and increased the levels of antioxidants. A total of 4,424 differentially expressed genes were responsive to cold, including 3,075 cold-induced and 1,349 cold-repressed genes. When cold stress was prolonged, there was an increase in the expression of cold-responsive genes coding for transporters, responses to stimuli and stress, regulation of defense response, as well as genes related to signal transduction of all phytohormones. Candidate genes in the terpenoid biosynthetic pathway were identified, eight of which were significantly involved in the cold stress response. Gene expression analyses using qRT-PCR showed a peak in the accumulation of SaCBF2 to 4, 50-fold more than control leaves and roots following 12 h and 24 h of cold stress, respectively. The CBF-dependent pathway may play a crucial role in increasing cold tolerance.

来源: Scientific Reports 浏览次数:0

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