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Sci Rep:中科院上海生科院张鹏研究组揭示木质素影响甘薯储藏根发育直接证据

摘要 : 2016年1月4日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表中国科学院上海生命科学研究院植物生理生态研究所张鹏研究组题为Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development 的研究论文。

 2016年1月4日,国际学术权威刊物自然出版集团旗下子刊《Scientific Reports》在线发表中国科学院上海生命科学研究院植物生理生态研究所张鹏研究组题为Altered Phenylpropanoid metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development 的研究论文。该研究工作揭示了木质素和淀粉竞争碳源,影响甘薯储藏根的发育。王红霞为论文第一作者,张鹏研究员为论文通讯作者。

甘薯是全球重要薯类作物,提供全球至少8亿人口的食品和工业原材料。储藏根发育的调控一直是备受关注的科学问题,深入了解储藏根发育机制对提高薯类产量具有重要意义。

张鹏研究组的王红霞等发现在紫薯中表达玉米转录因子Lc不仅提高了全株花青素的含量,而且影响储藏根的发育,形成类似“牛蒡根”的储藏根,薯型细长,产量及淀粉含量下降。进一步基因表达分析表明转基因植株储藏根中PAL表达显著上调,木质素合成基因4CL、C4H、CAD的表达也显著提升;木质素染色及含量测定确证了储藏根木质素的沉积。进一步分析发现转基因植株全株β-amylase的酶活明显提高,淀粉降解加速,叶片淀粉合成增强,说明通过加速叶片和储藏根中的淀粉降解可以补充根膨大所需的碳源。该研究为揭示木质素合成与储藏根发育的内在关系提供了新认识和思路。

木质素影响甘薯储藏根发育的直接证据

原文链接:

Altered Phenylpropanoid metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development

原文摘要:

There is no direct evidence of the effect of lignin metabolism on early storage root development in sweet potato. In this study, we found that heterologous expression of the maize leaf color (Lc) gene in sweet potato increased anthocyanin pigment accumulation in the whole plant and resulted in reduced size with an increased length/width ratio, low yield and less starch content in the early storage roots. RT-PCR analysis revealed dramatic up-regulation of the genes involved in the lignin biosynthesis pathway in developing storage roots, leading to greater lignin content in the Lc transgenic lines, compared to the wild type. This was also evidenced by the enhanced lignification of vascular cells in the early storage roots. Furthermore, increased expression of the β-amylase gene in leaves and storage roots also accelerated starch degradation and increased the sugar use efficiency, providing more energy and carbohydrate sources for lignin biosynthesis in the Lc transgenic sweet potato. Lesser starch accumulation was observed in the developing storage roots at the initiation stage in the Lc plants. Our study provides experimental evidence of the basic carbohydrate metabolism underlying the development of storage roots, which is the transformation of lignin biosynthesis to starch biosynthesis.

来源: Scientific Reports 浏览次数:0

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