nature

当前位置: Nature » 植物学 » 正文

Sci.Rep:华南植物园曾宋君研究组发表杏黄兜兰繁殖及其萌发机制研究进展

摘要 : 兜兰(Paphiopedilum)由于独特的花朵造型、绚丽的花朵色彩、持久的观赏花期而具有极高的观赏价值,在国际上广泛应用。由于生态环境的破坏以及人们对其过度地采挖,兜兰现已成为世界上最濒危的植物物种之一,许多种类已濒临灭绝,所有兜兰野生种均被列入《濒危野生动植物种国际贸易公约》(CITES)附录I而被禁止交易。

 兜兰(Paphiopedilum)由于独特的花朵造型、绚丽的花朵色彩、持久的观赏花期而具有极高的观赏价值,在国际上广泛应用。由于生态环境的破坏以及人们对其过度地采挖,兜兰现已成为世界上最濒危的植物物种之一,许多种类已濒临灭绝,所有兜兰野生种均被列入《濒危野生动植物种国际贸易公约》(CITES)附录I而被禁止交易。特别是仅产于我国云南的杏黄兜兰,是所有兜兰中唯一的黄色种类,被誉为“金童”,一经发现,便在国际园艺界引起轰动,在世界兰花展中多次获得金奖,国际上每株卖价曾高达8000美元。同时,杏黄兜兰又是所有兜兰植物中繁殖难度最大的种类之一,分株繁殖慢,成熟种子无菌萌发率也极低,是兜兰属植物繁殖和保护中的“旗舰”种类,可谓植物中的‘大熊猫’。

针对上述问题,华南植物园硕士研究生张艳艳在其导师曾宋君研究员、上海辰山植物园黄卫昌副园长等的指导下,对杏黄兜兰萌发困难的机制作了深入的研究并找到了解决其萌发困难的方法。实验结果表明:杏黄兜兰在成熟过程中经历了从低到高并急剧下降的萌发趋势;成熟种子无菌萌发率下降的原因与其种皮角质化形成不透水的保护层及其内部营养物质的消失具有重要的关系;选择合适胚龄的种子利用合适的培养基进行播种,利用合适浓度、合适时间的次氯酸钠处理成熟种子能有效地提高萌发率。

原文链接:

Embryo development in association with asymbiotic seed germination in vitro ofPaphiopedilum armeniacum S. C. Chen et F. Y. Liu

原文摘要:

This paper documents the key anatomical features during the development of P. armeniacum zygotic embryos and their ability to germinate asymbiotically in vitro. This study also examines the effect of media and seed pretreatments on seed germination and subsequent seedling growth. Seeds collected from pods 45 days after pollination (DAP) did not germinate while 95 DAP seeds displayed the highest seed germination percentage (96.2%). Most seedlings (50%) developed to stage 5 from 110 DAP seeds whose compact testa had not yet fully formed. Suspensor cells were vacuolated, which enabled the functional uptake of nutrients. The optimum basal medium for seed germination and subsequent protocorm development was eighth-strength Murashige and Skoog (1/8MS) for 95 DAP seeds and ¼MS for 110 DAP seeds. Poor germination was displayed by 140 DAP seeds with a compact testa. Pretreatment of dry mature seeds (180 DAP) with 1.0% sodium hypochlorite solution for 90 min or 40 kHz of ultrasound for 8 min improved germination percentage from 0 to 29.2% or to 19.7%, respectively. Plantlets that were at least 5 cm in height were transplanted to a Zhijing stone substrate for orchids, and 85.3% of plantlets survived 180 days after transplanting.

来源: Scientific Reports 浏览次数:0

热门文章TOP

RSS订阅 - 填写您的邮件地址,订阅我们的精彩内容: - 网站地图
网站联系电话:020-87540820 备案号:粤ICP备11050685号-8 增值电信业务经营许可证:粤B2-20120479
©2011-2015 生物帮 All rights reserved.