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Nat Com:抗体包裹的铁纳米粒子使干细胞靶向受损部位

摘要 : 美国洛杉矶Cedars-Sinai心脏研究所的研究人员使用两种抗体包覆铁纳米粒子,将其注入血液之后,这些粒子可使人体自身干细胞精确地定位于受损的心肌。相关文章发表于2014年9月10日的《Nature Communications》杂志上。
MagBICE纳米颗粒生产和体外结合性质

MagBICE纳米颗粒生产和体外结合性质

虽然干细胞可能是对抗某些疾病的有力武器,但是简单的用干细胞注入患者体内,不能保证干细胞将能够到达受损部位,并与已经存在于那里的细胞协同合作。

为了将能治愈的干细胞有针对性地传递到受伤部位,美国洛杉矶Cedars-Sinai心脏研究所的研究人员用两种抗体包覆铁纳米粒子,这两种抗体能够识别并特异性结合干细胞,并特异性结合体内的受损细胞。当纳米粒子注入到血液之后,它们成功地追踪到创伤部分并开始治疗。

该研究小组负责人、Cedars-Sinai 心脏研究所主任Eduardo Marbán博士说:“该结果是一种分子对接。通过磁共振成像,我们能够看到铁标记的干细胞,到达受损部位并且那里开始愈合。此外,通过在受损心脏上放置一块磁铁,可使铁纳米粒子更加接近受损心肌。”

作者认为,铁纳米粒子概念,也可以用来治疗其他健康问题,如外周血液疾病和哮喘。Marbán称,如果未来的研究是成功的,该程序可望在三年之内进行人体临床研究。

原文摘要:

Magnetic antibody-linked nanomatchmakers for therapeutic cell targeting

Ke Cheng, Deliang Shen, M. Taylor Hensley, Ryan Middleton, Baiming Sun, Weixin Liu,Geoffrey De Couto & Eduardo Marbán

Stem cell transplantation is a promising strategy for therapeutic cardiac regeneration, but current therapies are limited by inefficient interaction between potentially beneficial cells (either exogenously transplanted or endogenously recruited) and the injured tissue. Here we apply targeted nanomedicine to achieve in vivo cell-mediated tissue repair, imaging and localized enrichment without cellular transplantation. ​Iron nanoparticles are conjugated with two types of antibodies (one against antigens on therapeutic cells and the other directed at injured cells) to produce magnetic bifunctional cell engager (MagBICE). The antibodies link the therapeutic cells to the injured cells, wheras the ​iron core of MagBICE enables physical enrichment and imaging. We treat acute myocardial infarction by targeting exogenous bone marrow-derived stem cells (expressing ​CD45) or endogenous ​CD34-positive cells to injured cardiomyocytes (expressing myosin light chain. Targeting can be further enhanced by magnetic attraction, leading to augmented functional benefits. MagBICE represents a generalizable platform technology for regenerative medicine.

来源: Nature Communications 浏览次数:217

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